NF-kB p65 ELISA Kit

Produktnummer ABIN2044775

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human NF-kB p65.

Kurzübersicht für NF-kB p65 ELISA Kit (ABIN2044775)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails NF-kB p65 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Marke: BioAssay™

Sensitivität: < 0.053 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Andere Bezeichnung: V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA)

Pathways: NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine